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The aim of the study was to assess the effect of RFR on heat shock proteins. The authors studied the effects of RFR exposure on two cell lines - human glioblastoma A172, and human IMR-90 fibroblast. The cells were exposed to the W-CDMA cellular system, which is one of the component systems of the IMT-2000 system, and to continuous wave (CW) radiation. The A172 cells and IMP-90 fibroblasts were exposed to W-CDMA radiation at SARs of 0.08 and 0.8 W/kg for 2 hours. Next, the authors defined the cell doubling time of the A172 and IMR-90 cells, cultivated in 35mm culture dishes, to be 20-24 and 24-28 hours respectively. A172 cells were exposed to W-CDMA RFR at SARs of 0.08, 0.25, and 0.0 W/kg, and to CW RFR at 0.08 W/kg, for 24 or 48 hours (equalling one or two doubling times of the cells). IMR-90 cells were exposed to W-CDMA at 0.08 and 0.8 W/kg, and CW at 0.08 W/kg, for 28 hours, which is one doubling time of the cell. Sham exposure was also used The RF field exposure was assessed in a blind manner. HSP27 phosphorylation was assessed by bead-based multiplex assays, and gene expression of HSPs was assessed by DNA Chip analysis. No differences were noted between exposed and sham-exposed cells in phosphorylation levels or in gene expression of HSPs.
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