Brillaud E, Piotrowski A, de Seze R (2007)
The aim of this study was reproduce the observation made by Mausset-Bonnefont
and colleagues in 2004 that an acute exposure to RFR at 900 MHz
and SAR 0f 6 W/kg could induce astroglial activation 72 h later.
Eight groups of rats were studied. There were 4 groups of 9 animals
that were RFR-exposed for 15 minutes, 2 groups of sham-exposed
(6 per group), one negative control group to evaluate the potential
effect of containment, and one immunostaining positive control
group. One RFR exposed group was sacrificed at 2 days after exposure,
and the others at 3, 6 and 10 days post-exposure. One sham group
was sacrificed at 3 days and the other at 10 days after sham-exposure.
The brains were examined by an immunohistochemical method to assess
the amount of Glial Fibrillary Acidic Protein (GFAP), which appears
rapidly after astrocyte injury. Five areas of the brain were assessed
by analysts blind to the protocol.
A statistically significant increase in GFAP surface area was
seen in the frontal cortex and the caudate putamen 2 days after
RFR exposure, and after 3 days in the same areas and in the cerebellar
cortex. These effects were not seen after 6 or 10 days.
These results are in broad agreement with the results of Mausset-Bonnefont